Typical biochemical properties of Shigella spp. are:

lactose negative colonies (compare with E.coli)

D-mannitol fermentation (colour change around the mannitol tablet)

no acid from sucrose or cellobiose

urea hydrolysis negative (compare with K.pneumoniae)

acid from glucose without gas

hydrogen sulfide negative (compare with Salmonella enterica)

S.flexneri is ornithine negative (unlike S.sonnei)